ncRNA name | VEGF shRNA |
ncRNA Category | shRNA |
Disease name | choroidal neovascularization (CNV) |
Species | Homo sapiens |
Tissues/Cell_line | human embryonic retinoblast cell line 911, human RPE cell line ARPE-19 |
Methods | shRNA knockdown, fluorescence microscopy, qPCR, Northern blot, Western blot |
Expression pattern | associated |
Functional description | Potent shRNA sequences were identified that were able to silence VEGF in human RPE cells. When expressed from adenovirus backbones, these shRNA constructs silenced VEGF by 94% at a 1:5 molar ratio (VEGF to shRNA) and 64% at a 1:0.05 molar ratio. |
PubMed ID | 16877421 |
Year | 2006 |
Title | Inhibition of choroidal neovascularization by adenovirus-mediated delivery of short hairpin RNAs targeting VEGF as a potential therapy for AMD. |
Drug-related ncRNA | NA |
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